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Tissue Clearing Reagents

TP MedTech’s clearing reagents are based on our self-developed ionic liquid–based technology that achieves tissue transparency with minimal deformation and crystal-free preservation, while maintaining native structure. This overcomes common issues in conventional clearing, such as tissue distortion, signal loss, and ice-crystal damage.

Our reagents are compatible with multi-modal staining, allowing researchers to label carbohydrates, nucleic acids, and proteins in the same sample over multiple rounds, providing a solid foundation for high-quality imaging and following analysis.
  • Product Applications
  • Product Features
  • Product Comparison
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  • Frequently Asked Questions
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Product Applications

The VIVIT Tissue Clearing Kit provides an all-in-one solution for preparing ex vivo thick tissues for 3D imaging. It is compatible with a wide range of complex tissue types, enabling stable clearing of phospholipid-rich samples (such as brain tissue) and highly heterogeneous specimens (such as tumors).

Product Features
  • High transparency with minimal deformation
  • Enhanced fluorescence signals
  • Compatible with diverse 3D fluorescence imaging methods
  • Supports long-term storage at –80 °C without ice-crystal formation or tissue damage
  • Clears 1 mm–thick tissues within 24 hours
(Non-toxic and safe reagents)
Product Comparison

Product Name

 

VIVIT Tissue Clearing Kit

VIVIT Adipose-Rich Tissue Clearing Kit

VIVIT IF Tissue Clearing Kit

VIVIT IF Adipose-Rich Tissue Clearing Kit

 

Product Components
  • Delipidation reagent A1 (DIDC-S)
  • Overionization reagent A (SOS)
  • Ionic liquids matching reagents (RIL-S, IL5-S, IL2.5-S)
  • Delipidation reagent A1 (DIDC-S)
  • Delipidation reagent B
  • Overionization reagent B (SDS)
  • Ionic liquids matching reagents (RIL-S, IL5-S, IL2.5-S)
  • Antibody cross-linking reagent (10X)
  • Delipidation reagent A2 (DIDC-C)
  • Overionization reagent A (SOS)
  • Ionic liquids matching reagents (RIL-C, IL5-C, IL2.5-C)
  • Antibody cross-linking reagent (10X)
  • Delipidation reagent A2 (DIDC-C)
  • Delipidation reagent B
  • Overionization reagent B (SDS)
  • Ionic liquids matching reagents (RIL-C, IL5-C, IL2.5-C)

 

Application
  • Suitable for clearing non-adipose tissues such as the brain.
  • Enables visualization of endogenous fluorescent signals (e.g., fluorescent proteins).
  • Compatible with various 3D fluorescence imaging techniques.
  • Suitable for clearing adipose-rich tissues such as liver and lymph nodes.
  • Enables visualization of endogenous fluorescent signals (e.g., fluorescent proteins).
  • Compatible with various 3D fluorescence imaging techniques.
  • Suitable for clearing thick tissue slices after immunofluorescence staining.
  • Immunofluorescence staining can be performed using conventional protocols or thick-tissue methods (e.g., whole-mount, SWITCH, eFLASH).
  • Enables visualization of immunofluorescent signals in deep tissue.
  • Compatible with various 3D fluorescence imaging techniques.
  • Suitable for clearing adipose-rich tissue slices after immunofluorescence staining.
  • Immunofluorescence staining can be performed using conventional protocols or thick-tissue methods (e.g., whole-mount, SWITCH, eFLASH).
  • Enables visualization of immunofluorescent signals in deep tissue.
  • Compatible with various 3D fluorescence imaging techniques.

Packaging

10 mL × 5 vials

30 mL × 5 vials

10 mL × 6 vials

30 mL × 6 vials

10 mL × 5 vials

Plus 1 vial of antibody cross-linking reagent (10X)

 

30 mL × 5 vials

Plus 1 vial of antibody cross-linking reagent (10X)

 

10 mL × 6 vials

Plus 1 vial of antibody cross-linking reagent (10X)

 

30 mL × 6 vials

Plus 1 vial of antibody cross-linking reagent (10X)

 
Frequently Asked Questions
  • Do I need special containers for tissue clearing?
    No. Common laboratory containers made of polypropylene, polyethylene, or glassware can be used.
  • Can fresh tissue be cleared directly without fixation?
    No. Performing tissue clearing without fixation may cause disruption of the relative positions of cells and negatively affect subsequent procedures such as immunostaining. Therefore, samples must be fixed before clearing.
  • Can long-preserved fixed samples be used for tissue clearing?
    Yes. This product can be used for samples that have been immersed in fixative for several weeks, as well as samples that were fixed and stored at –80 °C for several months. However, if immunostaining is planned, long-term preserved fixed samples may exhibit reduced staining efficiency of target proteins.
  • How can I roughly estimate the amount of reagent needed for a sample?
    As an example, for a 1 mm-thick mouse brain slice, the recommended amount of each reagent is approximately 2 mL. When tissues contain large amounts of residual blood, high lipid content, or are thicker than average, the total reagent consumption may increase accordingly.
  • What is the refractive index (RI) of ionic liquid matching reagent IL5-S/IL5-C?
    The RI of IL5-S/IL5-C is 1.500–1.508. Do not mix IL5-S/IL5-C with other solvents (such as water), as this may alter the refractive index and compromise the observation of cleared samples.
  • What is the refractive index (RI) of ionic liquid matching reagent IL2.5-S/IL2.5-C?
    The RI of IL2.5-S/IL2.5-C is 1.538–1.545. Do not mix IL2.5-S/IL2.5-C with other solvents (such as water), as this may alter the refractive index and compromise the observation of cleared samples.
  • How long does the delipidation process take?
    For adult mouse brain slices, delipidation at 37 °C takes approximately 12–16 hours. For visceral tissues such as lung, heart, stomach, liver, and kidney, gradient delipidation is required, taking about 8–16 hours.
  • Can immunofluorescence-stained tissues be cleared?
    Yes. To clear immunofluorescence-stained tissues, please select the corresponding kit. It is also recommended to use DSG reagent for cross-linking after primary and secondary antibody staining.
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