Less than 1% deformation ensures subcellular structures remain intact.

Experimental data show that whole-brain samples treated with VIVIT exhibit minimal dimensional changes (–0.5% in length and –0.1% in width). The reagent has been validated across complex tissues including brain, multiple organs, and tumor models, and preserves subcellular structures such as synapses, providing a solid basis for 3D reconstruction and follow-up analysis.

VIVIT can optimize the fluorescent environment after tissue clearing. Its ionic liquid formulation enhances both protein- and dye-based fluorescence while suppressing bleaching, quenching, and diffusion, therefore lowering background fluorescence.
Experimental results show that VIVIT increases protein-based signals by 2–3 times and amplifies dye-based fluorescence even more substantially, with Cy3 enhanced up to 37-fold. By maintaining dye quantum efficiency, VIVIT improves the ratio of target signal to background fluorescence and ensures continuity, preventing discontinuities such as stitching artifacts during 3D reconstruction.

VIVIT uses a crystal-free vitrification approach that prevents the structural damage and signal loss typically caused by ice-crystal formation during freezing, while enabling long-term preservation at –80 °C.
Experimental results show that VIVIT-treated samples withstand at least three freeze–thaw cycles without visible damage and remain suitable for direct 3D imaging even after 13 months of continuous storage. This performance far surpasses PBS or sucrose-embedded tissues, making VIVIT ideal for multi-round imaging and large-scale analysis of valuable samples.